Figure 1. Average density (Bmax) maps for five 5-HT targets on the common FreeSurfer surface (left hemisphere; lateral view, upper and medial view, lower). Color scaling was individually adjusted in order to highlight features of the distributions.
Figure 2.Average density (Bmax) maps for the five 5-HT targets in the common MNI152 space (coronal, upper, z=8mm and sagittal, lower, x=-3mm). Color scaling was individually adjusted to highlight features of the distributions.
Figure 3. Density values (Bmax) of the five 5-HT targets in FreeSurfer defined brain regions. Median raphe is not reported for 5-HTT due the irreversible kinetic of the TACs, see also the Material and Methods section.
A table with Bmax values for all regions can be donwloaded here
Clustering of atlas maps
The abovementioned human brain atlas of the serotonin (5-HT) system does not conform with commonly used parcellations of neocortex, since the spatial
distribution of homogeneous 5-HT receptors and transporter is not aligned with such brain regions. This discrepancy indicates that a neocortical
parcellation specific to the 5-HT system is needed. Hence we present parcellations of the 5-HT system created using a clustering approach
focused on identifying stable and homogeneous clusters and derived from brain MR- and high-resolution PET images of five different 5-HT targets
from 210 healthy controls. This is the same data that was used in the derivation of the atlas above. The resulting parcellations show strong lateralization and do not indicate the presence of any network across the
combined 5-HT targets, with the exception of two segregated regions that share the same target profile. Furthermore, we reassess the known
regional associations between the density of 5-HT targets and mRNA levels and explore how well the parcellations can explain mRNA levels of 5-HT
related genes. The parcellations derived here represent a stable characterization of the 5-HT system which may be more sensitive than
traditional atlases to capture region-specific changes modulated by 5-HT.
Figure 4. Final clustering for K2 values of 2 (upper left), 7 (upper right), 18 (lower left) and 34 (lower right) for both the left and right hemispheres of the fsaverage5 in
ated surface. Rows 1 and 3 lateral views and rows 2 and 4 are medial views. Columns 1 and 3 are the left hemisphere and columns 2 and 4 are the right hemispheres. Colors between the left and right
hemispheres have been partially matched, however, each hemisphere should be considered independently from the other.
The following publications should be referenced when using this atlas:
A high-resolution in vivo atlas of the human brain's serotonin system
Vincent Beliveau, Melanie Ganz, Ling Feng, Brice Ozenne, Liselotte Højgaard, Patrick M. Fisher, Claus Svarer, Douglas N. Greve, Gitte M. Knudsen
J Neurosci. 2017 Jan 4; 37(1): 120 - 128.
The Structure of the Serotonin System: a PET Imaging Study
Vincent Beliveau, Brice Ozenne,Stephen Strother, Douglas N. Greve, Claus Svarer, Gitte M. Knudsen, Melanie Ganz
Bonaventure P, Hall H, Gommeren W, Cras P, Langlois X, Jurzak M, Leysen JE (2000) Mapping of serotonin 5-HT(4) receptor mRNA and ligand binding sites in the post-mortem human brain. Synapse 36:35-46.
Comtat C, Sureau FC, Sibomana M, Hong IK, Sjoholm N, Trebossen R (2008) Image based resolution modeling for the HRRT OSEM reconstructions software. In: 2008 IEEE Nuclear Science Symposium Conference Record, pp 4120-4123. IEEE.
Jovicich J, Czanner S, Greve D, Haley E, van der Kouwe A, Gollub R, Kennedy D, Schmitt F, Brown G, Macfall J, Fischl B, Dale A (2006) Reliability in multi-site structural MRI studies: effects of gradient non-linearity correction on phantom and human data. Neuroimage 30:436-443.
Knudsen GM et al. (2015) The Center for Integrated Molecular Brain Imaging (Cimbi) Database. Neuroimage:1-7.
Olesen OV, Sibomana M, Keller SH, Andersen F, Jensen J, Holm S, Svarer C, Højgaard L (2009) Spatial resolution of the HRRT PET scanner using 3D-OSEM PSF reconstruction. IEEE Nucl Sci Symp Conf Rec:3789-3790.
Sureau FC, Reader AJ, Comtat C, Leroy C, Ribeiro M-J, Buvat I, Trébossen R (2008) Impact of image-space resolution modeling for studies with the high-resolution research tomograph. J Nucl Med 49:1000-1008.
Varnäs K, Halldin C, Hall H (2004) Autoradiographic distribution of serotonin transporters and receptor subtypes in human brain. Hum Brain Mapp 22:246-260.
Woods RP, Cherry SR, Mazziotta JC (1992) Rapid automated algorithm for aligning and reslicing PET images. J Comput Assist Tomogr 16:620-633.
For questions related to the NRU serotonin atlas, contact Vincent Beliveau (vbeliveau-AT-nru.dk) or Melanie Ganz (mganz-AT-nru.dk).
Copyright (c) 2016, Neurobiology Research Unit, Rigshospital.
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